Encoding PCR Products with Batch-stamps and Barcodes
نویسندگان
چکیده
منابع مشابه
Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR.
PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular 'batch-stamps' that effectively label each genomic template with a sample ID and analysis date. This highly sensitive met...
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چکیده ندارد.
Directional cloning of PCR products.
INTRODUCTION This protocol is for directional blunt-end cloning of DNA fragments. The target DNA is PCR-amplified, 3'-extensions are polished with Pfu DNA polymerase, and the amplicon is ligated to a blunt-ended plasmid DNA. The products of the ligation reaction are used to transform competent Escherichia coli. A restriction enzyme is added to the ligation reaction (Liu and Schwartz 1992) to re...
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ژورنال
عنوان ژورنال: Biochemical Genetics
سال: 2007
ISSN: 0006-2928,1573-4927
DOI: 10.1007/s10528-007-9114-x